I have my ruby environment set up in .bash_profile and MacPorts installed in .profile, as per its instructions. I've installed node using MacPorts, but it seems like there's a conflict between .bash_profile and .profile. Node worked upon installation, but it hasn't worked since unless I delete .bash_profile or load .profile in the terminal.
I'm still new to setting up environments, so I'm not sure if I can essentially combine .bash_profile and .profile to avoid this conflict or if there's a better solution.
You can move the modifications MacPorts requires to $PATH wherever you want. If that fixes the issue, move them to .bash_profile.
Take a look at the value of $PATH when your setup works vs. when it doesn't. I assume whatever ruby environment you are using is changing it in some way. Adding the following line at the end of your .bash_profile should make it work again:
export PATH=/opt/local/bin:$PATH