Can you give an example of what might be best suited to place in the Application_Start and Session_Start subroutines? I know when each subroutine is called. Application_Start when the first user first accesses the web application. Session_Start when a user opens a session with the application. But what code belongs in each of these subroutines. What should the code in each subroutine do?
Just any examples? Well, in an MVC site the routes are registered in Application_Start. That's also a good place to initialize an IoC container such as StructureMap. Maybe initialize some singletons you have in your application if you want them to be readily available rather than late-bound when a user accesses them (like if they have a high initialization cost and you'd rather do one slow initial hit on the website yourself than bother a customer or two with it).
Session_Start is generally used less often, but could be a good place for per-user (or per-session, realistically) tracking of some kind.