image-processingimagejqupath

How to analyze-particles as beginner?

I am totally new in image analysis and have tried alot with ImageJ or QuPath but unfortunately I can’t find a proper way into it. Here is an image example I would like to quantify: enter image description here

Has anyone a recommendation which software I should use or how I can quantify those little “dots” also finding out their position?

I tried it with ImageJ, but the image quality is so bad that it does not allow thresholding…

With thresholding it seems impossible to quantify just the “little dots”…

Solution

  • result

    you can solve it in simple way using OpenCV, or you can go further with more sophisticated approach like this one.

    import cv2
import numpy as np

img = cv2.imread('img.png')

mask = cv2.cvtColor(img, cv2.COLOR_BGR2GRAY)
th = 35

mask[mask<th] = 0
mask[mask>0] = 255
mask = np.stack([mask, mask, mask], axis=2)

result = np.hstack((img, mask))

cv2.namedWindow("peaks", cv2.WINDOW_NORMAL)
cv2.imshow("peaks", result)
cv2.waitKey(0)
cv2.destroyAllWindows()