imagej

How can i analyze width and length of multiple plant pods in single image using ImageJ software?

Ihave tried to analyze the images of plant pods using ImageJ software but it does not show the width and length of a pod/pods accurately. Because the pods are curved, it gives the width of a pod upto 5 to 8 cm which should be below 1 cm. Can anyone suggest me exactly how we can measure the width and length of only black portions ?

Solution

  • Please provide a reference scale. Without it, your results will be in pixels not cm or mm!

    The rightmost pod results in the following values:

    OrthoMedial-Distances (widths)

    Mean = 52.65±8.39px;
Median = 55.97px;
MinMax = (10.5, 61.5)px;

    Medial-Axis

    Length = 1785.0px;

    EDIT: The analysis was done using the ImageJ-plugins "Easy_EdgeTrace.class" for tracing and and "general_TraceDistances.class" for the width and length evaluation. Both can be accessed from here. Make sure the pods are suitably oriented. The tracing won't work properly for pod #3. Here is what I get for the rightmost pod: Tracing & Medial Axis Here are the widths: Plot of the Widths

    OrthoMedial-Distances (widths):

    Mean = 0.69±0.11cm;
Median = 0.73cm;
MinMax = (0.13, 0.81)cm;

    Medial-Axis:

    Length = 23.90cm;