Best practice for using assert?

1. Is there a performance or code maintenance issue with using assert as part of the standard code instead of using it just for debugging purposes?

   Is

   assert x >= 0, 'x is less than zero'
   

   better or worse than

   if x < 0:
       raise Exception('x is less than zero')
   

2. Also, is there any way to set a business rule like if x < 0 raise error that is always checked without the try/except/finally so, if at anytime throughout the code x is less than 0 an error is raised, like if you set assert x < 0 at the start of a function, anywhere within the function where x becomes less then 0 an exception is raised?

To be able to automatically throw an error when x become less than zero throughout the function. You can use class descriptors. Here is an example:

class LessThanZeroException(Exception):
    pass

class variable(object):
    def __init__(self, value=0):
        self.__x = value

    def __set__(self, obj, value):
        if value < 0:
            raise LessThanZeroException('x is less than zero')

        self.__x  = value

    def __get__(self, obj, objType):
        return self.__x

class MyClass(object):
    x = variable()

>>> m = MyClass()
>>> m.x = 10
>>> m.x -= 20
Traceback (most recent call last):
  File "<stdin>", line 1, in <module>
  File "my.py", line 7, in __set__
    raise LessThanZeroException('x is less than zero')
LessThanZeroException: x is less than zero