I am currently using Primer3 2.3.6 in Linux. I am using the command "primer3_core -p3_settings_file=/my/path/to/settingsfile.txt /my/path/to/inputfile.txt".
I also tried it in Windows 7 and it worked without any problem. However, in Linux I got the error message "primer3_core: First line must be "Primer3 File - http://primer3.sourceforge.net"..". But the missing line is in the first line of my settings file.
Do you have any suggestions or ideas what could be wrong?
Thanks in advance!
Okay, I could solve the problem. It was more a mistake of myself, because I edited the default settings file, provided by primer3, using word pad in windows. Thereby, I changed the formatting and primer3 was not able to use it anymore. Now I just use the text editor or visual studio to change parameters in the settings file.