imagejfiji

remove or minimize the overlapping in image


I am working on a project where there is a high chance of overlapping particles in the images as partciles are coming down from the conveyor belt in the mining industry. I am also working on the hardware system to minimize the overlapping but none the less the images might have a certain level of overlapping. I am sharing my raw and processed image using imageJ Fiji. Please suggest me a good direction in this regards.Sample image after Processing in ImageJ And Raw Picture. My Goal is to find the size and shape of each particle. This is only possible if i successfully remove the overlapping between the particles. Thanks.


Solution

  • The particle splitting you are looking for is watershed analysis. Given that you seem to want detailed quantification in 2D, you might consider looking into the BioVoxxel plugin, which you can add to your installation of FIJI through the "Update..." command, then adding the update site.

    The plugin's ImageJ site is here: https://imagej.net/BioVoxxel_Toolbox

    It contains an extended particle analyzer (for 2D images), as well as a good watershed function, that should work for the shapes that you are trying to split. It may not split them perfectly, but if the overlap isn't too high, it should be sufficient for your purposes.