I Develop a flutter application, I am a Windows user. I don't have a MacBook, so is there any way to publish the flutter app on the app store using windows?
You can't build or publish iOS from Windows or any other system except MacOS. If you have no mac take a look at cloud services that can dedicate one to you (e.g. MacStadium). CI/CD services like CodeMagic, AppCenter is an option too, it is more suitable for small or pet projects when you don't need to build iOS often.